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sigmaplot 12.5 enzyme kinetics module  (SYSTAT)


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    SYSTAT sigmaplot 12.5 enzyme kinetics module
    Sigmaplot 12.5 Enzyme Kinetics Module, supplied by SYSTAT, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/enzyme+kinetics+module+of+sigmaplot+12%2E5/enzyme+kinetics+module+of+sigmaplot+12+5/pm36414408-108-16-21
    Average 90 stars, based on 1 article reviews
    sigmaplot 12.5 enzyme kinetics module - by Bioz Stars, 2026-09
    90/100 stars

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    Article Title: Functional characterization of 40 CYP2B6 allelic variants by assessing efavirenz 8-hydroxylation.
    Article Snippet: Genetic variations within cytochrome P450 2B6 (CYP2B6) contribute to inter-individual variation in the metabolism of clinically important drugs, including cyclophosphamide, bupropion, methadone and efavirenz (EFZ).. In this study, we performed an in vitro analysis of 40 CYP2B6 allelic variant proteins including seven novel variants identified in 1070 Japanese individuals.. Wild-type and 39 variant proteins were heterologously expressed in 293FT cells to estimate the kinetic parameters (Km, Vmax, and CLint) of EFZ 8-hydroxylation and 7ethoxy-4-trifluoromethylcoumarin (7-ETC) O-deethylation activities.

    Article Title: Functional Characterization of 21 Allelic Variants of Dihydropyrimidine Dehydrogenase Identified in 1070 Japanese Individuals.
    Article Snippet: The kinetic data were analyzed using the Enzyme Kinetics Module of SigmaPlot 12.5 (Systat Software, Inc., Chicago, IL, USA), a curve-fitting program based on nonlinear regression analysis.

    Article Title: Functional Assessment of 12 Rare Allelic CYP2C9 Variants Identified in a Population of 4773 Japanese Individuals
    Article Snippet: Kinetic parameters ( K m , k cat , V max , catalytic efficiency, and CL int ) were obtained using the Enzyme Kinetics Module of SigmaPlot 12.5 (Systat Software, Inc., Chicago, IL, USA), a curve-fitting program based on nonlinear regression analysis.

    Article Title: Interaction of the Sodium/Glucose Cotransporter (SGLT) 2 inhibitor Canagliflozin with SGLT1 and SGLT2.
    Article Snippet: All analyses were performed using the enzyme kinetics module of SigmaPlot 12.5 (Systat Software).

    Article Title: CYP2D6 genotyping analysis and functional characterization of novel allelic variants in a Ni-Vanuatu and Kenyan population by assessing dextromethorphan O-demethylation activity.
    Article Snippet: While CYP2D6 allele and phenotype frequencies have been extensively studied, currently, very little ethnically specific data is available regarding the East African and South Pacific region, including Kenya and Vanuatu.. The absence of information regarding gene polymorphisms and their resulting clinical effects in these populations may hinder treatment strategies and patient outcome.. Given the scarceness of CYP2D6 related data in these populations, the purpose of this study was to perform a pharmacogenomic analysis of the Kenyan and Ni-Vanuatu population and ultimately characterize the enzymatic properties of eight novel CYP2D6 variant proteins expressed in 293FT cells in vitro using dextromethorphan as a substrate.

    Article Title: Homologous expression and biochemical characterization of the arylsulfatase from Kluyveromyces lactis and its relevance in milk processing.
    Article Snippet: The industrial manufacturing process of lactosefree milk products depends on the application of commercial β-galactosidase (lactase) preparations.. These preparations are often obtained from Kluyveromyces lactis.. There is a gene present in the genome of K. lactis which should encode for an enzyme called arylsulfatase (EC 3.1.6.1).

    Article Title: Functional characterization of 9 CYP2A13 allelic variants by assessment of nicotine C-oxidation and coumarin 7-hydroxylation.
    Article Snippet: Cytochrome P450 2A13 (CYP2A13) is responsible for the metabolism of chemical compounds such as nicotine, coumarin, and tobacco-specific nitrosamine.. Several of these compounds have been recognized as procarcinogens activated by CYP2A13.. We recently showed that CYP2A13*2 contributes to interindividual variations observed in bladder cancer susceptibility because CYP2A13*2 might cause a decrease in enzymatic activity.

    Concentration Assay:

    Article Title: Transport of 3-fluoro-L-α-methyl-tyrosine (FAMT) by organic ion transporters explains renal background in [(18)F]FAMT positron emission tomography.
    Article Snippet: .. The transport rate was plotted against FAMT concentration and fitted to a MichaeliseMenten curve to obtain Michaelis constant (Km) andmaximal transport rate (Vmax) using enzyme kinetics module of SigmaPlot 12.5 (Systat Software, San Jose, CA). .. EadieeHofstee plot was also obtained using enzyme kinetics module of SigmaPlot 12.5 (Systat Software, San Jose, CA).



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